Chemistry of IGF-1 reconstitution question

Anubis

Hello fellas, its been a while. I've got a LR3 IGF-1 reconstitution question.

These are the reconstitution instructions that came with the IGF-1 (veteran members, i bet these instruction bring back memories):

"1. Draw 1mL solution and discard.
2.Use 2-3 ml water solution (only use water supplied) and measure vial dose accordingly. Continue to add the remaining solution.
3. DO NOT use benzyl alcohol (BA) or any other water to dilute.
4. Each vial will yield 1000mcgs (1mg) of IGF per vial. This yeilds 5mL @ 200 mcg/mL.
5. Inject water solution slowly-down the side of the vial DO NOT force.
6. Then slowly and gently twirl vial to mix solution. Allow solution to fully dissolve before commencing injections.
7. ONLY Inject via deep intramuscular (shoulders). Massage the injection area after each shot to allow for full absorbstion.
8. ONLY use insulin/diabetic needles.
9. Practice sterile injection procedures at ALL TIMES. Always swab both the top of vial and the local injection area before every injection.
10. MUST keep refridgerated VERY COLD at ALL TIMES once reconstituted (diluted).
11. Store at JUST above freezing temperature. DO NOT FREEZE.
12. Best temperature is just above 0 degrees Celsius.
13. Once reconstituted is stable at up to 8-12 weeks refridgerated very cold AT ALL TIMES (just above freezing temp).
14. Is stable at room temp in its dry state (before diluting) during shipping, due to this unique stability formula."


Like I said, it's been a while, and I need to walk through the science again and make sure I understand thoroughly before I start back up.

So it comes with a 0.9% NaCl solution, and the thought process behind using NaCl is that it has both the sufficient ionic strength to prevent agglutination and the requisite tonicity to prevent significant hemolysis/cell dehydration/ shrinkage, right? However, in return you're sacrificing any bacteriostatic and antipruritic properties. The instruction specifically advise against Benzyl Alcohol, and I'm like...why? Doesnt that sacrifice sterility? Would Benzyl Alcohol somehow denature the peptide and I'm just not understanding properly?


I remember alot of smart cookies on here a while back, hopefully some of you are still around.

__________________
The difference between Hanna Barbera and Hannibal the Barbarian.